Mō ngā tekau tau, kua whakawhirinaki te PDRN ki te tango mai i ngā pūtau whakaputa uri o te hāmana. He mea here tēnei ara tuku iho e ngā rerekētanga o te tuku ika, ngā raupapatanga DNA matapōkere, me ngā wero ki te whakahaere parakore—he mea uaua ki te kī taurangi te pumau mō te wā roa, te tauine, me te tutuki i ngā ture.
Tō mātouPDRN Whakahoui whakawhanakehia hei wikitoria i ēnei herenga hanganga mā te hangarau koiora matatau.
Kore rawa i ahu mai i ngā kararehe, i hangaia i runga i te koiora whakahaeretia
Mā te whakamahi i te E. coli DH5α hei tūāpapa whakaputa koiora, ka whakaurua ngā raupapa PDRN motuhake mā roto i ngā kawe recombinant, ā, ka tāruatia pai mā te whakapūtanga moroiti.
Mā tēnei huarahi ka whakakorea te whakawhirinaki ki ngā rauemi e ahu mai ana i te ika, ka aro atu ki te kore pumau o te tuku me ngā āwangawanga mō te haumaru o ngā kararehe i te pūtake, me te hangai anō ki ngā paerewa ture tino kaha i roto i te EU, te US, me ngā mākete o te ao.
I taua wā anō, ka noho tonu te huaHe mea hangai ki te DNA, ā, he mea hanga noa iho, e hanga ana heihe momo kē atu hei kai huawhenua, ehara i te kararehe, engari he mea tūturu ki te koioraki te PDRN tuku iho i ahu mai i te hāmana.
He raupapatanga tino hoahoa, ehara i te tangohanga matapōkere
He rerekē ki te PDRN tuku iho i whiwhihia mā te tangohanga kore-whiriwhiri, ka taea e te hangarau recombinant tete mana whakahaere katoa mō te raupapatanga DNA me te roa o te kongakonga.
Ka taea te hanga i ngā raupapa mekameka poto mō ngā tono ārai-mumura
Ka taea te whakarite i ngā raupapa mekameka waenga-ki-roa hei tautoko i te whakahoutanga o te collagen me te whakatikatika kiri.
Mā tēnei whakawhiti—mai i te tangohanga matapōkere ki te hanga koiora kua tohua—ka huaki ake ngā huarahi hou mō te whanaketanga e arohia ana e te mahi me ngā whakatakotoranga kua whakaritea.
Te tauine me te āheinga tāruarua o te taumata ahumahi
Mā te whakauru i te whakawhiti wera-ru kua arotauhia me te whakarite pūtau tino whai hua, ka tino pai ake te tango me te hua whakaputa plasmid.
I te whakakotahitanga me te kutikuti ā-tinana maha-taahiraa me te purenga chromatographic kua whakaritea, ka tutuki tonu i te tukangate parakore o te taumata hauora (≥99.5%).
Mā ngā tawhā whakapūmau paerewa ka pai ake te piki haere o te tauine mai i te whakaputanga whakamātautau ki te whakaputanga arumoni.
Te whai huatanga i whakamanahia e ngā raraunga haumanu-mua
E whakaatu ana ngā rangahau haumanu-mua ka tukuna e te PDRN recombinantte whakaohooho pai ake o te hanganga collagen Momo I tangataki te whakataurite ki te PDRN me ngā matatini DNA-konganuku i ahu mai i te hāmana tikanga.
Ka tautokohia e ēnei hua tōna whakamahinga hei whakatikatika kiri me te ārai i te koroheketanga, e tuku ana i tētahiotinga whakauru ka taea te whai raraunga, e ārahihia ana e te tikanga.
Ehara i te mea hei whakakapi noa iho te PDRN recombinant—he whakapai ake hangarau anō hoki.
Mā te whakakotahi i te hoahoa raupapatanga tika me te biosynthesis whakahaeretia, ka whakanuia e te hangarau recombinant te bioactivity PDRN me te whakarato i tehe huarahi kē atu pumau, huawhenua, me te taiaoki te PDRN i ahu mai i ngā kararehe—te whakatakoto i tētahi paearu hou mō ngā kai whakaora kiri o te whakatipuranga e whai ake nei.
Wā tuku: Hakihea-31-2025